Part:BBa_K1895006
Alternative electrically induced expression system using the dnaK promoter.
This is a composite part comprising a heat-shock promoter, dnaK, which drives expression of superfolder gfp when indduced with heat. The heat source can include the joule heating effect from an electric current.
Usage and Biology
DnaK is a heat shock protein. It's role is that of a protein chaperone, it binds to proteins that have misfolded due to the stress. These proteins are then refolded in an HSP-mediated process or degraded (Arsène F, et al., 2000). Like other members of the Hsp70 family, dnaK in E. coli is upregulated by heat stress (Ritossa, F., 1996). Consequently its promoter must be stronger during heat shock to allow for overexpression. Heat stress can be triggered by the joule effect heating produced by passing an electric current through the bacterial growth medium. This allows systems with dnaK as their promoter to be induced electrically.
This part is a variant on our 'lightbulb' construct (the previous variant using a different heat-shock promoter, htpG) which should cause the expression of super-folder gfp when an electric current is passed through the bacteria growth medium.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 221
| n/a | Alternative electrically induced expression system using the dnaK promoter. |